MVL Facilities

Virology and Molecular Biology:
Perkin Elmer 2000 PCR thermocycler; Heat Systems W-225R 200 W ultrasonicator with standard and microtip probes plus cup horn, Biorad 155 tube electrophoresis cell, Biorad tube gel destainer 172, Fisher FB GD 45 Gel Dryer, Lammert 10302 vacuum pump and cold trap, Fisher vacuum pump, Duerr vacuum pump, Biorad slab gel dryer 224, Biorad 221 vertical slab cell with cooling system, Power packs (Canalco, Biorad 400), Fisher FB 135 power pack, Hoefer Studier gel cells (SE400) (3 ea.), Hoefer PlateMate washer, UV Products transilluminator C61, Portable short-wave UV lamp, Hall productions light box, Beckman SW27, SW41 rotors, Submarine cell, Fisher Mini submarine cells (FB-MSU-1) (2 ea.), Fisher FB 105 power pack, Biorad Model 160/1.6 power pack, Polaroid CU5 camera and frame, Fisher Biotech DNA sequencing cells (FB-SEQ-3545) (2 ea.), Fisher FB 600 power pack, Fisher autoradiography cassettes 14x17 in. (5 ea.), Intensifying screens 14 x 17 in. (2 ea.), Fisher autoradiography cassettes 8 x 10 in. (5 ea.), Intensifying screens 8 x 10 in. (6 ea.), Boekel hybridizing ovens (5 cyl. capacity), Millipore 1225 sampling manifold, Amicon ultra filtration cells (200 ml, 50 ml, 10 ml), Eppendorf microcentrifuges (14,000 g) (2 ea.), Fisher MicroV variable-speed microfuge (7,200 g), Millipore peristaltic pump, National vacuum oven, EC microdensitometer with scanning software, Hoefer Mighty Small 4-gel cooled transphor unit, Fisher FB 570 power pack, Biorad Transblot apparatus, Biorad Bio-Dot Apparatus, BRL Hybri-dot blot manifold, Developing tanks and racks for X-ray films, Beckton-Dickinson Nutator mixer, Bellco rocker platform; Fisher Biotech gel photo documentation camera.

Cellular Biology:
IEC Model PR-J refrigerated centrifuge, IEC Model HN-S bench centrifuge, Olympus IX70 inverted phase-contrast microscope with 40X,20X, 10X, 4X objective and Nikon FM2 camera back; Olympus model CK inverted phase microscope with adapter and camera back; American Optical Phasestar phase contrast microscope; American Optical Phasestar dissecting microscopes (4 ea.); American Optical Phasestar bright field microscope; Bausch & Lomb dissecting microscope; Bausch & Lomb bright-field microscope; Nikon F70 camera back with Nikkor 28-80 zoom Lens mm and AF Micro Nikkor Lens 60 mm plus extension rings; copy stand; Linde XR-16 liquid nitrogen refrigerator and Dewar flask, Bellco platform rocker, Wheaton roller culture apparatus, Napco dual chamber CO2 incubator, Baker horizontal laminar-flow hood, ICC 6-foot vertical laminar-flow hood, Wescor 5100C vapor pressure osmometer, Lab-line Imperial II incubator.

General:
PowerMac G4 500 MHz, PowerBook G3, PowerBook 3400, PowerMac 8500, Power Macintosh 7100, LW 360 printer, LW310 printer, Epson Stylus Color 850 printer; UMAX Astra 1220S Scanner with UMAX UTA-3A transparency adapter; Epson ED-1200C scanner, several APS external drives, several Zip drives; Haake circulating water bath, Labline orbital shaker bath, VWR water bath, Napco water bath, Dry bath units (24-place) (4 ea.), Corning pH meters (2 ea.), Balance Denver Instruments XS 410 electronic balance; A&D HR-120 4-digit balance. Fisher scientific AB15 pH meter; Multi-Channel Pipettes: Rainin Pipetts: P-1000 3 ea.; P-200 3 ea.; P-100 2 ea.; P-20 5 ea.; P-10 1 ea.; Repeat pipettes (1 µl - 1.25 ml) 1 ea.; (P-200) 1 ea.; Fixed volume (10 µl) 1 ea., So-Low -80° freezer (5 cu. ft), So-Low -60° freezer (5 cu. ft), Freezer/refrigerators (4 ea.), Chest type freezer (25 cu. ft), Masterbuilt 36 sq. ft glass front cold cabinet, Vortex mixers, Magnetic stirrers, Uniden Geiger counter, Corning Mega-pure distillation apparatus, Continental de-ionizing system, 3 x 21 qt. pressure cookers, Sterilizing ovens (2 ea.), Napco drying oven, microwave ovens.

Dell Dimension 8200 Desktop: Intel^® Pentium^® 4 Processor at 1.8GHz, 256MB Dual Channel RDRAM, 21" Flat Panel displays, drive: 8x/4x/32x CD-RW/DVD. Dell Dimension 4400 Desktop: Intel^® Pentium^® 4 Processor at 1.6GHz, 256MB Dual Channel RDRAM, 19" Flat Panel displays, drive: 8x/4x/32x CD-RW/DVD. Dell Inspiron 8100: Intel^® Pentium^® 3 Processor at 1.2GHz, 512MB RAM, 15" displays monitor.

SHARED FACILITIES: General; Cell & Molecular Biology
MAINFRAME COMPUTERS

II. SHARED FACILITIES: (Biological Sciences; Center for Biotechnology & Genomics):

General:
Biology greenhouse: 8000 sq. ft., Biology walk-in environmental chambers: 8 x 100 sq. ft, Biology USDA-approved animal room: 1500 sq. ft, Microbiology cold room space: 300 sq. ft., Biology cold room space: 4 x 100 sq. ft., Microbiology stockroom/prep-room (800 sq. ft), Electron microscopy (800 sq. ft.), Walk-In incubators (300 sq. ft.). Several automatic washers, Several dryers, Several autoclaves, Departmental water purification system (reverse osmosis plus deionized water supplied to all labs)

Cell & Molecular Biology:
Beckman J2-21 centrifuge with JA20, JS7, and JS13 rotors plus four Sorvalls, Beckman L 3-50 ultracentrifuge with SW27, SW41, and type 70 rotors plus two additional ultracentrifuges, Beckman LS 6500 multipurpose bench top scintillation counter (new), Beckman LS 7500 scintillation counter, Beckman DU-64 UV spectrophotometer, Packard Biosciences Cobra II Series Cobra Quantum Gamma counter, Scanning electron microscope, two Transmission electron microscopes, Ultra-microtome, Freeze-fracture machine, and Pharmacia FPLC system. Additional advanced equipment is available at the Biotechnology and Genomics Core Facilities operated by the Institute for Biotechnology and Genomics. These include: Beckman Gold HPLC system, Milligen 6625 Pro-Sequencer, Milligen Sy-Clone DNA synthesizer, Laser densitometer, Thermocyclers (5),a BioRad Particle Gun, three chromatogranphy systems (a Pharmacia FPLC, Perkin-Elmer Biosystems Biocad Sprint, and a Dionex HPLC), Automatic X-ray developing system, Imagegraphics computerized slide-making system (Macintosh & Windows), (6) Texas Tech University has recently acquired five additional DNA auto-sequencers at the Institute for Biotechnology and Genomics.

MVL: Virology and Molecular Biology; Cellular Biology; General
MAINFRAME COMPUTERS

III. TEXAS TECH ACADEMIC COMPUTER SYSTEM:

VAX and IBM, UNIX, and Sun systems; Access to Texas Tech University Library Catalog; Access to literature databases through Texas Tech University Library; First Search (Medline, Agricola, BasicBIOSIS, etc.); UnCover; Ideal (On-line journals); Access to Current Contents (disk form) through department; WEB GCG (Wisconsin Computer Genetics Group; WCGG); Access through internet to DNA and protein analysis tools; National Center for Biotechnology Information (NCBI); European Bioinformatics Institute (EBI).

MVL: Virology and Molecular Biology; Cellular Biology; General
SHARED FACILITIES: General; Cell & Molecular Biology